Awarded by by Eolicas do Sul, a subsidiary of national power company Eletrobras, the contract includes supplying 72 of Gamesa's G97 2MW machines.
Gamesa said that it will supply, install and commission the turbines at six wind farms being developed in the southern Brazilian state Rio Grande do Sul.
In addition to this, the company will also conduct civil engineering works and provide operation and maintenance services for 15 years.
The wind turbines, which will be manufactured at Gamesa's local Camaçari factory, are planned to be delivered during the third quarter of 2014.
The wind farm is expected to be commissioned during the first quarter of 2015.
According to Gamesa, the latest contract is the second between the company and Eolicas do Sul following a contract at the end of 2012 for 258MW of turbines at ten wind farms in Brazil.
Since the commencement of its operations in Brazil, Gamesa has secured firm orders for more than 1,400MW.
South Cone managing director Edgard Corrochano said: "This new contract consolidates Gamesa as one of the leading turbine makers in Brazil, a position underpinned by the combination of our global technological prowess with local know-how, coupled with our commitment to community development, creating jobs and generating wealth through local supply chain development."
The company has installed more than 1,900 MW of turbines in Latin American nations, including Mexico, Honduras, Argentina and Costa Rica.
Image: Gamesa wins 144MW wind turbine supply contract in Brazil. Photo: courtesy of Gamesa.